|
|
|
|
|
| C O N T E N T S |
| C-Fern Home |
| Web Manual |
| Introduction C-Fern Nutrient Medium Culture Conditions Gametophyte Culture Gametophyte Observations Sporophyte Culture Spore Mutagenesis Bibliography |
|
Light Stand Growth Pod DNA Extraction C-Fern T-shirt Who's Who Selection and mutants Media formulation error Image gallery |
| Web Journal |
| Educational Resources |
|
National Science Standards Gametophyte development Student research questions Research in the classroom Research with C-Fern Brief description of C-Fern C-Fern manipulation Root tropism? |
| Frequently Asked Questions |
| Workshop and Exhibit Schedule |
| Educational
Materials |
| Investigations Mutant & wild type spores Manual & Culture Instructions Supplies - Domes, media |
|
 |
| Development
of C-Fern is supported by the National Science Foundation (NSF-DUE) |
|
|
|
Copyright
© 1997-2000
Thomas R. Warne and Leslie G. Hickok. All rights reserved. |
|
|
C-Fern
Sport Report
This mutation, which was induced by X-rays, confers tolerance to 2'-deoxy-5-fluorouridine (FUdR). This substance is a pyrimidine nucleoside analog and is highly toxic to many organisms. As a nucleotide analog, FUdR interferes with normal metabolism and nucleic acid biosynthesis. This mutation confers a very clear tolerance to FUdR and when gametophytes carrying it are grown in medium containing 10 mM FUdR, wild type gametophytes are killed shortly after germinating while mutant gametophytes grow quite normally. Wu, K. and J. King. 1994. Biochemical and genetic characterization of 5-fluro-2'-deoxyuridine-resistant mutants of Arabidopsis thaliana. Planta 194: 117-122. |
