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• 3D Reconstruction of the Antennal Lobe of Drosophila melanoga
• 3D Reconstruction of the Antennal Lobe of Drosophila melanogaster We present the first three-dimensional map of the antennal lobe of D. melanogaster, based on confocal microscopic analysis of glomeruli stained with the neuropil-specific monoclonal antibody nc82. The analysis of confocal stacks allowed us to identify glomeruli according to the criteria shape, size, position and intensity of antibody labeling. Forty glomeruli were labeled by nc82, eight of which have not been described before. Three glomeruli previously shown exclusively by backfills were not discernible in nc82 stainings. Six glomeruli consist of distinct, but contiguous structural units, termed 'compartments'. Glomerular variability observed occasionally between males and females is in the same range as between individuals of the same sex, suggesting the lack of a significant sexual dimorphism in the glomerular pattern. An important goal of this work was to create these 3D reference models of the antennal lobe, which are all accessible on-line.
• FlyBase A Database of the Drosophila Genome
• Annotated Horizontal Autofluorescent Sections Sixteen 7 µm horizontal paraffin sections of male flies. Autofluorescence was imaged using a confocal microscope at 1024 x 1024 resolution. Images are provided in JPEG format here, a series of high resolution GIF versions is also available. Horizontal sections are arranged from dorsal to ventral.
• Berkeley Drosophila Genome Project
• Flybrain-Java Browse through the brain of a fly by Java The browser allows the user to quickly manipulate a set of serial sections, with gridlines, annotations, text, and full size diagrams. This work is currently under development, to create faster, more user-friendly applications.
• Central Complex Architectures
• Characterization of Gal4 lines with expression in the Drosophila optic lobe usin We have used the Gal4/UAS targeted expression system to analyze the regional specificity and developmental dynamics of reporter gene expression in a collection of enhancer trap lines. These lines express the yeast transcription factor Gal4 in subsets of cells within the optic lobe of Drosophila. As a reporter, we used a tau-GFP fusion construct (1). This fusion protein is transported into axonal processes due to the microtubuli-binding properties of the tau protein. It offers a high level of resolution in structural analysis. Due to its natural fluorescence, it is ideally suited for combination with antibody stains for two-channel confocal laser scan microscopy.
• Chun-Fang Wu genetic control of function and development of the nervous system
• Columnar lamina-medulla connections in structural brain mutants This poster describes some important facets of the small optic lobes and irreC phenotypes by use of the Golgi method. Emphasis is on the columnar neurons that connect retina and lamina with the medulla.
• Computational requirements for Flybrain
• Das Fischbach Labor
• Descending Neurons of the Vertical Cell and Ocellar System (DNOVS)
• Doe lab For those of you unfamiliar with our work, we work on CNS development in Drosophila
• Douglas Kankel seeks a definition of the molecular mechanisms which underlie the assembly of the central nervous system
• Educational Tour of the Drosophila Nervous System
• eyeless-Fehlexpressionsphänotypen Daten und Bilder aus: Georg Halder, Patrick Callaerts und Walter Gehring (1995). Induction of Ectopic Eyes by Targeted Expression of the eyeless Gene in Drosophila. Science 267, 1788-1792
• FlyBase (a Drosophila genome database)
• FlyBase FlyBase archives at the EBI, UK
• FlyBase FlyBase, a Database of the Drosophila Genome
• FLYBRAIN
• Flybrain, an Online Atlas and Database of the Drosophila Nervous System
• Flybrain, an Online Atlas and Database of the Drosophila Nervous System
• Flybrain, an Online Atlas and Database of the Drosophila Nervous System
• Flybrain, an Online Atlas and Database of the Drosophila Nervous System
• Flybrain, an Online Atlas and Database of the Drosophila Nervous System
• FlyView - a Drosophila image database FlyView is the beginning of an image database on Drosophila development and genetics, especially on expression patterns of genes (enhancer trap lines, cloned genes).The concept of FlyView includes compatibility to FlyBase, the main Drosophila database.
• GAL4-responsive UAS-tau as a tool for studying the anatomy and
• Giant Fibre Pathway
• Images of the Drosophila Nervous System
• Informationsquellen zu Drosophila melanogaster
• IrreC-rst misexpression in central brain structures does not alter their develop IrreC-rst, a transmembrane protein of the immunoglobulin superfamily with 5 immunoglobulin-like domains, is required for normal axonal projections in both optic chiasms (Boschert et al., 1990; Ramos et al., 1993). Normally it is not expressed in developing central complex structures. When misexpressed in these structures (by use of the Gal4/UAS-system; Brand and Perrimon, 1993), it does not seem to affect their development. We cloned the irreC-rst cDNA HB3 into the pUAST vector, behind UAS binding sites for the yeast transcription factor Gal4 and established five independent UAS-HB3 transformant lines, that were subsequently crossed to Gal4-lines. The IrreC-rst protein in F1 hybrids was detected by mab24A5.1 (Schneider et al., 1995) which is directed against the extracellular domain of IrreC-rst. We viewed pupal brain whole mounts in the confocal microscope.
• John A. Pollock focusses on the molecular genetics of the neural networks required for vision
• Kalpana White studies functions crucial to neuronal maturation during embryogenesis and in the neurons of the visual system
• Linking Neuroblasts to Their Corresponding Lineage Development of the central nervous system (CNS) in Drosophila starts with the delamination from the neuroectoderm of about 30 neuroblasts (NBs) per hemineuromere, giving rise to approximately 330 - 350 neurons and 30 glial cells. Understanding the mechanisms leading to cell fate specification and differentiation in the CNS requires the identification of the NB lineages. Here we present the combined data from Bossing et al. (1996) and Schmidt et al. (1997), i.e. 29 embryonic NB lineages. With one exception we were able to link these lineages to the corresponding NBs. Two NBs give rise to glial progeny exclusively (GP, NB 6-4A), seven NB lineages are composed of glial cells as well as neurons (NB 1-1A, 1-3, 2-2T, 2-5, 5-6, 6-4T and 7-4) while the other NB lineages only contain neuronal cells.
• Nobel Prize in Physiology or Medicine 1995 Announcement by the Nobel Assembly
• Nüsslein-Volhard Lab (MPI für Entwicklungsbiologie)
• Optic Lobe Development in 3rd Instar and Early Pupal Stages The resources provided here are intended to enable researchers to compare enhancer trap or gene expression patterns to reference stainings and to clarify the complex topology of the early optic lobe. The developing lamina is the projection area of the ingrowing photoreceptors. The actual lamina neuropil is only a very thin layer in the larval stage. The lamina surrounds the inner cell plug, which derives from the inner optic anlage. The lamina itself is proliferated from the outer optic anlage (OOA). The Bolwig nerve connects the larval photoreceptors with the larval optic neuropil, which is situated at the base of the medulla. The inner optic anlage (IOA) is the proliferation region which creates neurons of the lobula and proximal medulla. The distal medulla neuropil is the projection area of long retinula cells R7 and R8 and of lamina monopolar neurons. Here connections are made to dendrites of transmedulla neurons derived from the OOA. The proximal medulla neuropil contains the aborizations of neurons formed by the IOA as well as branches of transmedulla neurons that project to the lobula complex. Those cells that project from the lobula complex neuropils to central brain regions are derivatives of the IOA. The inner cell plug built by the IOA contains the population of T and C cells.
• Overview of Flybrain
• Gene Networks Overview of the Drosophila Segmentation Gene Networks Gap-genes, segmentation genes, dorsal, torso ...
• Renditions of 3D Structures
• Serge Côté Laboratory (Quebec) Genetic and molecular analysis of embryonic development in Drosophila
• Submission and Citation of Data
• Synaptic neuropils of the Drosophila brain
• The Drosophila mushroom body is a quadruple structure of clonal units The mushroom body (MB) is an important centre for higher-order sensory integration and learning in insects. To analyse the development and organisation of the MB neuropile in Drosophila, we performed cell lineage analysis in the adult brain with a new technique that combines the flippase (flp) / FRT system and the GAL4 / UAS system (FRT-GAL4 system) (Fig. 2). We showed that the four mushroom body neuroblasts (MBNbs) give birth exclusively to the neurones and glial cells of the MB, and that each of the four MBNb clones contributes to the entire MB structure (Fig. 3). The expression patterns of 19 GAL4 enhancer-trap strains that mark various subsets of MB cells revealed overlapping cell types in all four of the MBNb lineages (Fig. 4 and Fig. 5). Partial ablation of MBNbs using hydroxyurea showed that each of the four neuroblasts autonomously generates the entire repertoire of the known MB substructures (Fig. 6). Thus, we conclude that the MB is a four-fold structure of clonal units each of which contains an essentially identical set of neurones and glial cells (summarised in Fig. 1).
• The Flybrain 3D-Project
• GIFTS The Gene Interactions in the Fly Trans-World Server
• The Interactive Fly A cyberspace guide to Drosophila genes and their roles in development
• The Major Brain Centers
• Thomas Schmidt-Glenewinkel Research Interest: Biochemistry and Molecular Biology of Neurotransmitter Receptors and Ion Channels in Drosophila Melanogaster
• What's New in Flybrain