C-Fern Logo  C-Fern
C O N T E N T S
C-Fern Home
Web Manual
  Introduction
  C-Fern Nutrient Medium
  Culture Conditions
  Gametophyte Culture
  Gametophyte Observations
  Sporophyte Culture
  Spore Mutagenesis
  Bibliography

Other Resources

  Light Stand
  Growth Pod
  DNA Extraction
  C-Fern T-shirt
  Who's Who
  Selection and mutants
  Media formulation error
  Image gallery
Web Journal
Educational Resources
  National Science Standards
  Gametophyte development
  Student research questions
  Research in the classroom
  Research with C-Fern
  Brief description of C-Fern
  C-Fern manipulation
  Root tropism?
Frequently Asked Questions
Workshop and Exhibit Schedule
 Educational Materials
  Investigations
  Mutant & wild type spores
  Manual & Culture Instructions
  Supplies - Domes, media
Have questions?
Contact the C-Fern Project
cfern@utk.edu
 Supported, in part, by the National Science Foundation - Division of Undergraduate Education
Development of C-Fern is supported
by the National Science Foundation (NSF-DUE)
Copyright © 1997-2000
Thomas R. Warne and
Leslie G. Hickok.
All rights reserved.
 C-Fern Sport Report

 
Hermaphroditic and male C-Fern gametophytes
pale (pal1)


Ghost-like pal1 gametophytes appear to have decreasing amounts of chlorophyll as they enlarge. Nonetheless, they grow to sexual maturity within two weeks and can self or cross-fertilize. At 1-2 weeks, pal1 gametophytes are slightly smaller than wild type and their pale phenotype makes them clearly recognizable in a segregating population (use of transmitted light is best). This is a recessive mutation in sporophytes and leaves of pal1/pal1 homozygotes show a yellow –green phenotype when viewed with reflected light (from the side or top). These attractive sporophytes are quite viable, but slower growing than wild type. This EMS-induced mutation is available only in an F1 hybrid stock. The F1 spores can be used very effectively in the Genetics in Action Kit (15-6708), as a substitute for polka dot (cp).