C-Fern Logo  C-Fern
C O N T E N T S
C-Fern Home
Web Manual
  Introduction
  C-Fern Nutrient Medium
  Culture Conditions
  Gametophyte Culture
  Gametophyte Observations
  Sporophyte Culture
  Spore Mutagenesis
  Bibliography

Other Resources

  Light Stand
  Growth Pod
  DNA Extraction
  C-Fern T-shirt
  Who's Who
  Selection and mutants
  Media formulation error
  Image gallery
Web Journal
Educational Resources
  National Science Standards
  Gametophyte development
  Student research questions
  Research in the classroom
  Research with C-Fern
  Brief description of C-Fern
  C-Fern manipulation
  Root tropism?
Frequently Asked Questions
Workshop and Exhibit Schedule
 Educational Materials
  Investigations
  Mutant & wild type spores
  Manual & Culture Instructions
  Supplies - Domes, media
Have questions?
Contact the C-Fern Project
cfern@utk.edu
 Supported, in part, by the National Science Foundation - Division of Undergraduate Education
Development of C-Fern is supported
by the National Science Foundation (NSF-DUE)
Copyright © 1997-2000
Thomas R. Warne and
Leslie G. Hickok.
All rights reserved.

C-Fern Educational Kits

Genetics in Action - Mendelian Genetics Kit (15-6750)

  Hermaphroditic and male C-Fern gametophytes
 
C-Fern Educational Materials are available from Carolina Biological Supply Company. For information please contact Carolina Biological at http://www.carolina.com or
call: 800-334-5551
fax 800-222-7112.
 
C-Fern Educational Kits

Students visualize basic principles of Mendelian Inheritance in C-Fern by following the segregation of a visible marker, polka dot, in both the F1 gametophyte and F2 sporophyte generations. Students sow spores of an F1 hybrid (wild type X polka dot) to produce F1 gametophytes. By adding water to mature F1 gametophytes, students visualize random fertilization events that produce the F2 sporophyte generation. Hypotheses generated from observations of F1 gametophytes are then tested by analysis of phenotypes and ratios in the F2 sporophyte generation. The large sample numbers provided from class and individual data sets allow meaningful use of the Chi-square test. This investigation demonstrates the Mendelian principles of segregation, random fertilization and dominance/recessiveness and provides hands-on experience in data acquisition, hypothesis formation and testing. Materials for 30 individuals. Experiment time is 14 -28 days.

  • *1 Vial Presterilized F1 (wild type X polka dot) C-Fern (Ceratopteris) Spores
  • *3 Bottles C-Fern Basic Medium
  • *40 Sterile Petri Dishes 60 x 15mm
  • *Sterile Water
  • *10 Sterile Pipets
  • Pen
  • Culture Dome
  • *10 Sterile Spore Spreaders

15-6751. Refurbishment Set for 15-6750. Includes all items with *.