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Development
of C-Fern is supported by the National Science Foundation (NSF-DUE) |
Copyright
© 1997-2000
Thomas R. Warne and Leslie G. Hickok. All rights reserved. |
C-Fern
Web Manual - Spore Mutagenesis
Materials:
Procedures:
1. Soak spores for 24 h prior to sterilization and irradiation.
2. Sterilize using standard methods and place sterile spore suspension in
a glass petri dish. Place dish inside plastic sandwich bag.
3. Irradiate spores with 25,000 Roentgens or about 555 Roentgens min-1 for
45 min. After irradiation sow spores as usual.
Under these conditions, spore germination at 10 days following sowing is
about 80%; a screen with 10-5 M flurodeoxyuridine gives a mutant frequency
of 2 X 10-5.
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