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| C O N T E N T S |
| C-Fern Home |
| Web Manual |
| Introduction C-Fern Nutrient Medium Culture Conditions Gametophyte Culture Gametophyte Observations Sporophyte Culture Spore Mutagenesis Bibliography |
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Light Stand Growth Pod DNA Extraction C-Fern T-shirt Who's Who Selection and mutants Media formulation error Image gallery |
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| Educational Resources |
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National Science Standards Gametophyte development Student research questions Research in the classroom Research with C-Fern Brief description of C-Fern C-Fern manipulation Root tropism? |
| Frequently Asked Questions |
| Workshop and Exhibit Schedule |
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| Investigations Mutant & wild type spores Manual & Culture Instructions Supplies - Domes, media |
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| Development
of C-Fern is supported by the National Science Foundation (NSF-DUE) |
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Copyright
© 1997-2000
Thomas R. Warne and Leslie G. Hickok. All rights reserved. |
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C-Fern
Web Manual - Culture of Gametophytes
Temperature The temperature optimum for spore germination and gametophytic development is about 28 C (82 F) as measured inside the Culture Dome or C-Fern Growth Pod. This is somewhat higher than for many other plants. Similar growth and development of gametophytes can be obtained at 26-30 C (79-86F). However, lower temperatures will substantially alter developmental timing; for example, at 20 C (68 F) development times will be increased by about two-fold. It is a good idea to monitor and record the temperature inside Growth Pods or Culture Domes daily. Some control of the temperature inside the Growth Pods or Culture Domes can be achieved by adjusting the distance between the light source and C-Fern cultures. Once a suitable temperature is achieved, the height of the lights should remain constant during all phases of culture. |
