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Die molekulare Grundlage der Synapsenbildung an der neuromuskulären
Endplatte u
Fast alle Untersuchungen zur molekularen Grundlage der Synaptogenese wurden bisher an einer speziellen Synapse außerhalb des Gehirns gemacht, nämlich der neuromuskulären Endplatte in der Skelettmuskulatur von Vertebraten. An dieser speziellen Synapse ist einer der Hauptregulatoren das extrazelluläre Matrixprotein Agrin. Während der Entwicklung wird Agrin in den Zellkörpern von Motoneuronen im ventralen Horn des Rückenmarks synthetisiert und entlang des Axons zur präsynaptischen Nervenendigung transportiert. Dort wird Agrin freigesetzt und bindet einerseits an eine dünne Schicht aus extrazellulärer Matrix, einer sogenannten Basalmembran, und induziert andererseits auf der Muskelfaser die Aggregation der Neurotransmitterrezeptoren sowie anderer Proteine.
- Characterization of Gal4 lines with expression in the Drosophila optic lobe usin
We have used the Gal4/UAS targeted expression system to analyze the regional specificity and developmental dynamics of reporter gene expression in a collection of enhancer trap lines. These lines express the yeast transcription factor Gal4 in subsets of cells within the optic lobe of Drosophila. As a reporter, we used a tau-GFP fusion construct (1). This fusion protein is transported into axonal processes due to the microtubuli-binding properties of the tau protein. It offers a high level of resolution in structural analysis. Due to its natural fluorescence, it is ideally suited for combination with antibody stains for two-channel confocal laser scan microscopy.
- Clean-up team (acetylcholinesterase)
Discovery: Insights through advanced computing, Cornell Theory Center
- GABAA receptor research
The group of Erwin Sigel. Research on the GABAA receptor and on the benzodiazepine binding site
- IrreC-rst misexpression in central brain structures does not alter their develop
IrreC-rst, a transmembrane protein of the immunoglobulin superfamily with 5 immunoglobulin-like domains, is required for normal axonal projections in both optic chiasms (Boschert et al., 1990; Ramos et al., 1993). Normally it is not expressed in developing central complex structures. When misexpressed in these structures (by use of the Gal4/UAS-system; Brand and Perrimon, 1993), it does not seem to affect their development. We cloned the irreC-rst cDNA HB3 into the pUAST vector, behind UAS binding sites for the yeast transcription factor Gal4 and established five independent UAS-HB3 transformant lines, that were subsequently crossed to Gal4-lines. The IrreC-rst protein in F1 hybrids was detected by mab24A5.1 (Schneider et al., 1995) which is directed against the extracellular domain of IrreC-rst. We viewed pupal brain whole mounts in the confocal microscope.
- John A. Pollock
focusses on the molecular genetics of the neural networks required for vision
- MDC Max Delbrück Centrum für Molekulare Medizin, Berlin
- SFB391 Mechanismen der schnellen Zellaktivierung, München
- SFB317 Molekulare Biologie neuraler Mechanismen und Interaktionen
- Universität Bochum, Lehrstuhl für Molekulare Neurobiochemie
- ZMNH Zentrum für Molekulare Neurobiologie, Hamburg
© 2000 by Kurt Stüber