ENTRY       EC 3.4.99.44
NAME        Pitrilysin
            E. coli protease
            Protease Pi
CLASS       Hydrolases
            Acting on peptide bonds (peptidases)
            Endopeptidases of unknown catalytic mechanism
REACTION    Preferential cleavage of Tyr16+Leu and Phe25+Tyr bonds of oxidised
            insulin B chain.  Also acts on other substrates of Mr less than 7
            kDa such as insulin and glucagon.
SUBSTRATE   Protein
            Peptide
            H2O
PRODUCT     Protein
            Peptide
INHIBITOR   EDTA
            1,10-Phenanthroline
COMMENT     From the periplasmic space of E. coli.  Inhibited by EDTA and
            1,10-phenanthroline; not thiol-dependent.  A homologue of
            insulinase.
DBLINKS     University of Geneva ENZYME DATA BANK: 3.4.99.44
            WIT (What Is There) Metabolic Reconstruction: 3.4.99.44
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