ENTRY       EC 3.4.24.44
NAME        Atrolysin E
            Crotalus atrox metalloendopeptidase e
            Hemorrhagic toxin e
CLASS       Hydrolases
            Acting on peptide bonds (peptidases)
            Metalloendopeptidases
REACTION    Cleavage of Asn3+Gln, Ser9+His and Ala14+Leu bonds in insulin B
            chain and Tyr14+Gln and Ala8+Ser in A chain. Cleaves type IV
            collagen at Ala258+Gln in alpha1(IV) and at Gly191+Leu in
            alpha2(IV).
SUBSTRATE   Protein
            Peptide
            H2O
PRODUCT     Protein
            Peptide
COFACTOR    Metal
COMMENT     A 25.7 kDa hemorrhagic endopeptidase from the venom of the
            western diamondback rattlesnake (Crotalus atrox) that digests
            basement membrane components, including the triple helix of
            type IV collagen. Such action is believed to contribute to the
            hemorrhagic property by weakening capillary walls.
MOTIF       PS: PS00142  [GSTALIVN]-x(2)-H-E-[LIVMFYW]-{DEHRKP}-H-x-
                         [LIVMFYWGSPQ]
            PS: PS00427  C-x(2)-G-x-C-C-x-[NQRS]-C-x-[FM]-x(6)-C-[RK]
DBLINKS     University of Geneva ENZYME DATA BANK: 3.4.24.44
            WIT (What Is There) Metabolic Reconstruction: 3.4.24.44
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