ENTRY       EC 3.4.24.3
NAME        Microbial collagenase
            Clostridium histolyticum collagenase
            Clostridiopeptidase A
            Collagenase A
            Collagenase I
            Achromobacter iophagus collagenase
CLASS       Hydrolases
            Acting on peptide bonds (peptidases)
            Metalloendopeptidases
REACTION    Digestion of native collagen in the triple helical region at +Gly
            bonds. With synthetic peptides, a preference is shown for Gly at
            P3 and P1'; Pro and Ala at P2 and P2'; and hydroxyproline, Ala or
            Arg at P3'
SUBSTRATE   Collagen
            H2O
PRODUCT     Protein
            Peptide
COMMENT     Six species of metalloendopeptidase acting on native collagen can
            be isolated from the medium of Clostridium histolyticum. Class I
            has forms alpha (68 kDa), beta (115kDa) and gamma (79 kDa);
            class II has delta (100 kDa), epsilon (110 kDa) and xi (125 kDa).
            The two classes are immunologically crossreactive, but have
            significantly different sequences, and different specificities
            such that their actions on collagen are complementary. The enzymes
            also act as peptidyl-tripeptidases. Variants of the enzyme have
            been purified from Bacillus cereus, Empedobacter collagenolyticum,
            and V. alginolyticus (previously Achromobacter iophagus). Also
            known from Streptomyces sp. Formerly EC 3.4.99.5, 3.4.4.19 and
            3.4.24.8.
MOTIF       PS: PS00142  [GSTALIVN]-x(2)-H-E-[LIVMFYW]-{DEHRKP}-H-x-
                         [LIVMFYWGSPQ]
DBLINKS     University of Geneva ENZYME DATA BANK: 3.4.24.3
            WIT (What Is There) Metabolic Reconstruction: 3.4.24.3
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