ENTRY       EC 3.4.23.32
NAME        Scytalidopepsin B
            Scytalidium aspartic proteinase B
CLASS       Hydrolases
            Acting on peptide bonds (peptidases)
            Aspartic endopeptidases
REACTION    Hydrolysis of proteins with broad specificity, cleaving
            Phe24+Phe, but not Leu15-Tyr and Phe25-Tyr in the B chain of
            insulin.
SUBSTRATE   Protein
            Peptide
            H2O
PRODUCT     Protein
            Peptide
COMMENT     A second endopeptidase from Scytalidium lignicolum (see
            scytalidopepsin A) that is insensitive to pepstatin and methyl
            2-diazoacetamidohexanoate. 1,2-Epoxy-3-(p-nitrophenoxy)propane
            reacts with Glu53, which replaces one of the aspartic residues at
            the active centre. One of the smallest aspartic endopeptidases
            active as the monomer, with Mr 22 kDa. Similarly inhibitor-
            -resistant endopeptidases are found in the basidiomycetes
            Lentinus edodes and Ganoderma lucidum, and in Polyporus
            tulipiferae (a second endopeptidase distinct from
            polyporopepsin), but these are of typical aspartic endopeptidase
            size.
DBLINKS     University of Geneva ENZYME DATA BANK: 3.4.23.32
            WIT (What Is There) Metabolic Reconstruction: 3.4.23.32
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