ENTRY EC 3.4.22.34
NAME Legumain
Bean endopeptidase
Vicilin peptidohydrolase
Phaseolin
CLASS Hydrolases
Acting on peptide bonds (peptidases)
Cysteine endopeptidases
REACTION Hydrolysis of proteins, such as azocasein. Preferential cleavage:
Asn+ in small molecule substrates such as Boc-Asn+OPhNO2
SUBSTRATE Protein
Peptide
H2O
Boc-Asn-OPhNO2
PRODUCT Protein
Peptide
Boc-Asn
HOPhNO2
COMMENT From germinating cotyledons of mung bean (Vigna radiata) and
garden bean (Phaseolus vulgaris). Thought to be involved in the
hydrolysis of stored seed proteins.
DBLINKS University of Geneva ENZYME DATA BANK: 3.4.22.34
WIT (What Is There) Metabolic Reconstruction: 3.4.22.34
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