ENTRY       EC 3.4.22.32
NAME        Stem bromelain
CLASS       Hydrolases
            Acting on peptide bonds (peptidases)
            Cysteine endopeptidases
REACTION    Broad specificity for cleavage of proteins, but strong preference
            for Z-Arg-Arg+NHMec amongst small molecule substrates
SUBSTRATE   Protein
            Peptide
            Z-Arg-Arg-NHMec
            H2O
PRODUCT     Protein
            Peptide
            Z-Arg-Arg
            NH2Mec
COMMENT     The most abundant of the cysteine endopeptidases of the stem of
            the pineapple plant, Ananas comosus.  Distinct from the bromelain
            found in the pineapple fruit (EC 3.4.22.33).  Scarcely inhibited
            by chicken cystatin, and also very slowly inactivated by E-64.
            Formerly EC 3.4.4.24 and included in EC 3.4.22.4.
MOTIF       PS: PS00139  Q-x(3)-[GE]-x-C-[YW]-x(2)-[STAGC]-[STAGCV]
            PS: PS00639  [LIVMGSTAN]-x-H-[GSACE]-[LIVM]-x-[LIVMAT](2)-G-x-
                         [GSADNH]
            PS: PS00640  [FYCH]-[WI]-[LIVT]-x-[KRQAG]-N-[ST]-W-x(3)-[FYW]-G-
                         x(2)-G-[LFYW]-[LIVMFYG]-x-[LIVMF]
DBLINKS     University of Geneva ENZYME DATA BANK: 3.4.22.32
            WIT (What Is There) Metabolic Reconstruction: 3.4.22.32
///