ENTRY EC 3.2.1.68
NAME Isoamylase
Debranching enzyme
CLASS Hydrolases
Glycosidases
Hydrolysing O-glycosyl compounds
SYSNAME Glycogen 6-glucanohydrolase
REACTION Hydrolysis of 1,6-alpha-D-glucosidic branch linkages in glycogen,
amylopectin and thier beta-limit dextrins
SUBSTRATE Glycogen
Amylopectin
beta-Limit dextrin
H2O
PRODUCT D-Glucose
COMMENT Distinguished from alpha-dextrin endo-1,6-alpha-glucosidase
(EC 3.2.1.41) by the inability of isoamylase to attack pullulan,
and by limited action on alpha-limit dextrins. Action on
glycogen however is complete in contrast to limited action by
alpha-dextrin glucanohydrolase. 1,6-Linkage hydrolysed only if at
a branch point.
DBLINKS University of Geneva ENZYME DATA BANK: 3.2.1.68
WIT (What Is There) Metabolic Reconstruction: 3.2.1.68
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