ENTRY       EC 3.1.27.3
NAME        Ribonuclease T1
            Guanyloribonuclease
            Aspergillus oryzae ribonuclease
            RNase N1 and N2
CLASS       Hydrolases
            Acting on ester bonds
            Endoribonucleases producing other than 5'-phosphomonoesters
REACTION    Two-stage endonucleolytic cleavage to 3'-phosphomononucleotides
            and 3'-phosphooligonucleotides ending in Gp with 2',3'-cyclic
            phosphate intermediate
SUBSTRATE   RNA
            H2O
PRODUCT     3'-Phosphomononucleotide
            3'-Phosphooligonucleotide
COMMENT     Similar enzymes: N.crassa RNase N1 and N2; Ustilago sphaerogena
            RNase; Chalaropsis RNase; B.subtilis RNase; Microbial RNase I.
            Formerly EC 2.7.7.26 and 3.1.4.8.
            Hydrolyzes the R(p)-configuration of phosphorothioate isomer of
            oligoribonucleotides containing phosphorothioate-linkage almost
            exclusively. Review: 'Stereochemistry of enzymatic reaction of
            phosphates', P.A.Frey, Tetrahedron 38, 1541 (1982).
STRUCTURES  PDB: 1FUS  1DET  1BIR  1RGF  1RGE  1FUT  1GMP  1GMQ  1RGC  1GMR  
                 1LRA  1RCK  1RCL  1RDS  1RGA  1RGG  1RGK  1RGL  8RNT  7RNT  
                 6RNT  5RNT  4RNT  3RNT  2RNT  2BIR  2AAE  2AAD  1TRQ  1TRP  
                 1RSN  1RNT  1RN4  1RN1  1RGH  1RLS  9RNT  
DBLINKS     University of Geneva ENZYME DATA BANK: 3.1.27.3
            WIT (What Is There) Metabolic Reconstruction: 3.1.27.3
            SCOP (Structural Classificaion of Proteins): 3.1.27.3
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