ENTRY EC 3.1.21.5
NAME Type III site-specific deoxyribonuclease
Type III restriction enzyme
Restriction-Modification system
CLASS Hydrolases
Acting on ester bonds
Endodeoxyribonucleases producing 5'-phosphomonoesters
REACTION Endonucleolytic cleavage of DNA to give specific double-stranded
fragments with terminal 5'-phosphates
SUBSTRATE DNA
H2O
PRODUCT DNA 5'-phosphate
COFACTOR ATP
S-Adenosyl-L-methionine
COMMENT This is a large group of enzymes which, together with those now
listed as EC 3.1.21.3 and 3.1.21.4, were previously listed
separately in sub-subclasses 3.1.23 and 3.1.24. They have an
absolute requirement for ATP, but do not hydrolyse it;
S-Adenosyl-L-methionine stimulates the reaction, but is not
absolutely required. They recognize specific short DNA sequences
and cleave a short distance away from the recognition sequence.
These enzymes exist as complexes with enzymes of similar
specificity listed under EC 2.1.1.72 or 2.1.1.73. A complete
listing of all these enzymes has been produced by R.J.Roberts;
this list is updated annually.
DBLINKS University of Geneva ENZYME DATA BANK: 3.1.21.5
WIT (What Is There) Metabolic Reconstruction: 3.1.21.5
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