ENTRY       EC 1.3.99.8
NAME        2-Furoyl-CoA dehydrogenase
            Furoyl-CoA hydroxylase
CLASS       Oxidoreductases
            Acting on the CH-CH group of donors
            With other acceptors
SYSNAME     2-Furoyl-CoA:(acceptor) 5-oxidoreductase (hydroxylating)
REACTION    2-Furoyl-CoA + H2O + Acceptor = S-(5-Hydroxy-2-furoyl)-CoA +
            Reduced acceptor
SUBSTRATE   2-Furoyl-CoA
            H2O
            Acceptor
            Methylene blue
            Nitro blue
            Tetrazolium
PRODUCT     S-(5-Hydroxy-2-furoyl)-CoA
            Reduced acceptor
COFACTOR    Copper
COMMENT     A copper protein.  The exygen atom of the -OH produced is derived
            from water, not O2; the actual oxidative step is probably
            dehydrogenation of a hydrated form -CHOH-CH2- to -C(OH)=CH-,
            which tautomerizes non-enzymically to -CO-CH2-, giving
            (5-oxo-4,5-dihydro-2-furoyl)-CoA.  Methylene blue, nitro blue,
            tetrazolium and a membrane fraction from P. putida can act as
            acceptors.
DBLINKS     University of Geneva ENZYME DATA BANK: 1.3.99.8
            WIT (What Is There) Metabolic Reconstruction: 1.3.99.8
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