ENTRY       EC 1.14.15.1
NAME        Camphor 5-monooxygenase
            Camphor 5-exo-methylene hydroxylase
CLASS       Oxidoreductases
            Acting on paired donors with incorporation of molecular oxygen
            With a reduced iron-sulfur protein as one donor, and
            incorporation of one atom of oxygen
SYSNAME     (+)-Camphor,reduced-putidaredoxin:oxygen oxidoreductase
            (5-hydroxylating)
REACTION    (+)-Camphor + Putidaredoxin + O2 = (+)-exo-5-Hydroxycamphor +
            Oxidized putidaredoxin + H2O
SUBSTRATE   (+)-Camphor
            Putidaredoxin
            O2
            (-)-Camphor
            1,2-Campholide
PRODUCT     (+)-exo-5-Hydroxycamphor
            Oxidized putidaredoxin
            H2O
            5-exo-Hydroxy-1,2-campholide
COFACTOR    Heme
COMMENT     A heme-thiolate protein (P-450)
            Also acts on (-)-camphor and 1,2-campholide, forming
            5-exo-hydroxy-1,2-campholide.
            #STRUCTURE:
            Helcies E(149-156), F(176-185), G(192-205), and I(234-267) form an
            antiparallel Greek key helical bundle.
            Heme: the heme is embedded between the proximal (helix L) and
            distal (helix I) helicies. The heme propionates interact with
            Arg112, Arg299, and His355. Gly249 is close approach to the distal
            heme surface. Gly249 is conxerved among other P-450.
            Axial heme ligand: Cys357 of the N-terminal end of helix L binds
            to Fe(III) in the high-spin state. Phe350, Leu358, and Gln360 form
            a pocket for Cys357.
            Camphor: The carbonyl oxygen form hydrogen bonding to Tyr96 and
            makes hydrophobic contact with Phe87, Leu244, Val247, Thr252,
            Val295. Tyr96 is unique to P-450cam.
            (Poulos et al. J.Biol.Chem., 260, 16122-16130, 1985)
MOTIF       PS: PS00086  F-[SGNH]-x-[GD]-x-[RHPT]-x-C-[LIVMFAP]-[GAD]
STRUCTURES  PDB: 1NOO  1PHA  1PHB  1PHC  1PHD  1PHE  1PHF  1PHG  2CP4  2CPP  
                 3CP4  3CPP  4CP4  4CPP  5CPP  6CPP  7CPP  8CPP  1CP4  
DBLINKS     University of Geneva ENZYME DATA BANK: 1.14.15.1
            WIT (What Is There) Metabolic Reconstruction: 1.14.15.1
            SCOP (Structural Classificaion of Proteins): 1.14.15.1
///