ENTRY       EC 1.14.13.52
NAME        Isoflavone 3'-hydroxylase
CLASS       Oxidoreductases
            Acting on paired donors with incorporation of molecular oxygen
            With NADH or NADPH as one donor, and incorporation of one atom of
            oxygen
SYSNAME     Formononetin,NADPH:oxygen oxidoreductase (3'-hydroxylating)
REACTION    Formononetin + NADPH + O2 = Calycosin + NADP+ + H2O
SUBSTRATE   Formononetin
            NADPH
            O2
PRODUCT     Calycosin
            NADP+
            H2O
COFACTOR    Heme
COMMENT     A heme-thiolate protein (P-450). Also acts on biochanin A and
            other isoflavones with a 4'-methoxy group. Involved in the
            biosynthesis of the pterocarpin phytoalexins medicarpin and
            maachiain.
DBLINKS     University of Geneva ENZYME DATA BANK: 1.14.13.52
            WIT (What Is There) Metabolic Reconstruction: 1.14.13.52
///