Botany online 1996-2004. No further update, only historical document of botanical science!

Melting of DNA (Thermal Denaturation)

abscissa: temperature, ordinate: relative UV-absorption. The transition from one conformation (double strand) to another (single strand) goes along with an increase in absorption. The Tm-value (blue line) corresponds to the temperature at which half of the molecules is single and the other half double stranded.

© Peter v. Sengbusch -