MCB 229 Spring 2000 Study Guide 2 Prof. Terry
Covers Lecture for Feb. 3

This study guide is intended for you to use while you are doing the assigned text reading. Quiz questions will be made with reference to topics in this study guide. This guide covers assigned reading for Feb. 3. Note: Quiz #2, based on questions from this study guide, must be completed by midnight before the class on Thurs. Feb. 3. You will need to create your "myWebCT" account and visit the MCB 229 WebCT page in order to access this quiz.

Chapter 3 (pp. 51-69)
  1. What is peptidoglycan (a.k.a. murein)? What is the envelope? What is the difference?
  2. Where is peptidoglycan found in a gram-negative cell? In a gram-positive cell? Which cell has the thicker peptidoglycan layer? Examine Fig. 3.19 and be able to tell the difference between gram-negative and gram-positive walls visually.
  3. What is the periplasmic space? In which type of bacteria is it mainly found? What kinds of enzymes are found in this region?
  4. What are the 2 sugars found in peptidoglycan? What are the peptide components, and what is unusual about them? How many amino acids are attached to a sugar?
  5. What is a "peptide interbridge", and which type of bacteria is it typically found? (see fig. 3.22)
  6. In which types of bacteria are are teichoic acids found?
  7. In which types of bacteria is there an outer membrane? What unusual lipid components are found in this membrane? What does LPS stand for? What are the components of LPS?
  8. What happens during plasmolysis?
  9. What effect does the enzyme lysozyme have on bacterial cells?
  10. What effect does penicillin have on bacterial cells?
  11. What is a spheroplast (also known as a protoplast)? What happens to spheroplasts in distilled water?
  12. What is the difference between a slime layer and a capsule? Where are these structures found? (Note that both of these can also be called glycocalyx.)
  13. What do fimbriae look like? How do they differ in appearance from flagella?
  14. What is the difference between a "monotrichously flagellated" cell, a "lophotrichously flagellated" cell, and a "peritrichously flagellated" cell?
  15. How is a bacterial flagellum attached to the cell? (see fig. 3.37) How does it move so as to cause motility?
  16. What is meant by "positive chemotaxis"? What is meant by "negative chemotaxis"? What is a "run"? What is a "twiddle" (also called a "tumble")?
  17. We'll talk about more details of chemotactic motility in class -- don't worry for now about the details on page 65-66.
  18. What are special properties of the bacterial endospore? How resistant are endospores to heat?
  19. What unusual chemicals are found in the endospore wall? (see fig 3.46).
  20. What is the difference between "sporulation" and "germination"?