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of C-Fern is supported by the National Science Foundation (NSF-DUE) |
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Copyright
© 1997-2000
Thomas R. Warne and Leslie G. Hickok. All rights reserved. |
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Mutant Selection
The identification of an appropriate selection condition typically requires careful dose response curves to determine the correct concentration of a toxin (for example, selections using herbicides, FuDR, etc.) that results in a sufficient inhibition of gametophyte growth or development. Several factors may need to be considered when choosing the appropriate selection regime, for example: The nature of the toxic response, e.g. whether abrupt or gradual The pattern of gametophytic growth and development over time, e.g. whether gametophytes exhibit differential response dependent upon age. Identification of phenotypes other than growth that may identify mutants Also for identification of putative morphological mutants (for example clumped chloroplast or callus-like growth habit) careful observation of gametophytes at a sufficiently young stage is important. The standard selection procedure is outlined in this illustration.(Figure not yet available!) An example of a successful selection using media supplemented with FuDR is illustrated below. There are about 12,000 spore on this dish and all except one are severly inhibited or killed by this concentration of FuDR. The single individual (right, central region of dish) exhibits tolerance to FuDR and has nearly normal growth and development. A number of confirmed and putative mutants have been obtained using both X-ray and EMS mutagenesis.
Examples of mutant selections of Ceratopteris
Hickok and Schwarz, 1986a,b,
1989; Carrol et al. 1988 Hickok et al. 1987 and
unpublished Tai Chun and Hickok 1992 Hickok et al. 1987; Warne
and Hickok1987; Hickok et al. 1991; Vogelien et al., 1993; Warne
et al. 1995 Singh, 1990 Hickok et al.1987 Hickok et al. 1987 Hickok et al. 1987 Wright et al. 1990 Warne et al. 1988; Banks
et al.1993; Banks 1994 Hickok 1985a; Banks 1994 Scott and Hickok 1991;
Cooke et al.1987, 1993 Cooke et al. 1995 Hickok et al. 1995 Vaughn et al., 1990
(1) All listed selections, with the exception of 230X, were derived
from the Hn-n strain. Strain designations ending with 'n' indicate that
a number of independent selections are available, all of which have not
been fully characterized genetically.
Complete information on references can be found in the Ceratopteris
Bibliography |
