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| C O N T E N T S |
| C-Fern Home |
| Web Manual |
| Introduction C-Fern Nutrient Medium Culture Conditions Gametophyte Culture Gametophyte Observations Sporophyte Culture Spore Mutagenesis Bibliography |
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Light Stand Growth Pod DNA Extraction C-Fern T-shirt Who's Who Selection and mutants Media formulation error Image gallery |
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| Educational Resources |
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National Science Standards Gametophyte development Student research questions Research in the classroom Research with C-Fern Brief description of C-Fern C-Fern manipulation Root tropism? |
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| Investigations Mutant & wild type spores Manual & Culture Instructions Supplies - Domes, media |
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| Development
of C-Fern is supported by the National Science Foundation (NSF-DUE) |
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Copyright
© 1997-2000
Thomas R. Warne and Leslie G. Hickok. All rights reserved. |
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C-Fern
Web Manual - Culture of Gametophytes
Spore Germination and Gametophyte Development Under standard temperature and light conditions, spore germination is evident three to four days following spore soaking (DFS) and complete by six to eight DFS. Male and hermaphroditic gametophytes are sexually mature at 10 to 12 DFS. This is the best time to observe fertilization or to perform cross or self fertilization for sporophytes. In older gametophytes effective spermatozoid production decreases. |
