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| C O N T E N T S |
| C-Fern Home |
| Web Manual |
| Introduction C-Fern Nutrient Medium Culture Conditions Gametophyte Culture Gametophyte Observations Sporophyte Culture Spore Mutagenesis Bibliography |
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Light Stand Growth Pod DNA Extraction C-Fern T-shirt Who's Who Selection and mutants Media formulation error Image gallery |
| Web Journal |
| Educational Resources |
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National Science Standards Gametophyte development Student research questions Research in the classroom Research with C-Fern Brief description of C-Fern C-Fern manipulation Root tropism? |
| Frequently Asked Questions |
| Workshop and Exhibit Schedule |
| Educational
Materials |
| Investigations Mutant & wild type spores Manual & Culture Instructions Supplies - Domes, media |
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| Development
of C-Fern is supported by the National Science Foundation (NSF-DUE) |
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Copyright
© 1997-2000
Thomas R. Warne and Leslie G. Hickok. All rights reserved. |
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C-Fern
Web Manual - Culture of Gametophytes
Light C-Fern Culture Domes - Continuous illumination by two 40 W cool white fluorescent tubes at a distance of 45 cm or less from the cultures (about 80 umoles photosynthetically active radiation m-2 sec-1) will accommodate two standard Culture Domes (54 X 27 cm). This will provide enough space for up to 64 individual 60x15 mm Petri dishes. Smaller or larger setups can be used to serve individual needs. C-Fern Growth Pods - For each Pod, use continuous illumination by a 15 watt screw-in fluorescent bulb placed in a small (ca. 6") reflective dome fixture. Each Pod can accomodate 30 individual 60x15 mm Petri dishes. Temperature inside Growth Pods and Culture Dome is more important than light level, so the distance between the Culture Dome and the light source can be varied considerably to obtain a temperature near the optimum. |
