ENTRY       EC 3.1.21.5
NAME        Type III site-specific deoxyribonuclease
            Type III restriction enzyme
            Restriction-Modification system
CLASS       Hydrolases
            Acting on ester bonds
            Endodeoxyribonucleases producing 5'-phosphomonoesters
REACTION    Endonucleolytic cleavage of DNA to give specific double-stranded
            fragments with terminal 5'-phosphates
SUBSTRATE   DNA
            H2O
PRODUCT     DNA 5'-phosphate
COFACTOR    ATP
            S-Adenosyl-L-methionine
COMMENT     This is a large group of enzymes which, together with those now
            listed as EC 3.1.21.3 and 3.1.21.4, were previously listed
            separately in sub-subclasses 3.1.23 and 3.1.24. They have an
            absolute requirement for ATP, but do not hydrolyse it;
            S-Adenosyl-L-methionine stimulates the reaction, but is not
            absolutely required. They recognize specific short DNA sequences
            and cleave a short distance away from the recognition sequence.
            These enzymes exist as complexes with enzymes of similar
            specificity listed under EC 2.1.1.72 or 2.1.1.73. A complete
            listing of all these enzymes has been produced by R.J.Roberts;
            this list is updated annually.
DBLINKS     University of Geneva ENZYME DATA BANK: 3.1.21.5
            WIT (What Is There) Metabolic Reconstruction: 3.1.21.5
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