ibclogo XVI International Botanical Congess

Abstract Number: 6111
Session = 1.3.5


GENETIC ANALYSIS OF PLANT SYMBIOSES WITH RHIZOBIUM AND MYCORRHIZAE


Cook, D.1, Penmetsa, V.1, Denarie, J.2, Gough, C.2, Blaylock, L.2, Harrison, M.J.3, Krishnamurthy, N.1, and Vanden Bosch, K.A.1, 1 Texas A&M University, College Station, TX, 2Laboratoire de Biologie Moleculaire, CNRS-INRA, Castanet-Tolosan, France, 3 The Samuel Roberts Noble Foundation, Ardmore, OK


We used a visual assay to screen EMS mutagenized seedling populations for nodulation mutants. Approximately one-half of the selected M2 individuals were classified as non-nodulators (nod-). To subdivide this potentially large phenotypic class, we assayed bacterial infection by means of an S. meliloti symbiont transformed with a constitutively expressed lacZ fusion. Based on the extent of rhizobial infection (inf) and the ability to make nodule primordia (pri), we have identified three classes of nod minus mutants: 1) inf-/pri-, 2) inf+/pri-, and 3) inf-/pri+. Each of these classes of mutants have also been analyzed for mycorrhizal associations.

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