ibclogo XVI International Botanical Congess

Abstract Number: 6047
Session = 3.15.6


MINISATELLITE DNA VARIATION AMONG AND WITHIN APOMICTIC BLACKBERRY SPECIES (RUBUS SUBGEN. RUBUS)


H. Nybom*, T. Kraft#, and K. Antonius€ (*Balsgård-Dept. Of Horticultural Plant Breeding, Swedish University of Agricultural Sciences, Kristianstad, Sweden, #Dept. Of genetics, Lund University, Lund Sweden, €Dept. of Plant Biology and Plant Breeding, Helsinki, Finland)


Most blackberry species in Rubus subgen. Rubus are polyploid, facultatively apomictic and pseudogamous. Minisatellite probe hybridization, using mainly the M13 repeat sequence, proved very efficient for discrimination of genotypes and was used to study genetic variation in some European blackberry species. No intra-populational variation was found, and intraspecific variation was very limited. Some genotypes cover large area and occur in several countries. Intraspecific variation appears to be even more limited in N Europe that in C Europe, suggesting a pattern of geographic parthenogenesis. The low levels of genetic variation encountered in parthenogenesis. The low levels of genetic variation encountered in natural populations is specially puzzling since M13-probing of offspring derived from interspecific cross-pollination experiments reveal approx. 10% sexual seed set. Some of the interspecific hybrids reproduce apomictically whereas others completely sexual, suggesting a simple mode of inheritance of apomixis.

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