ibclogo XVI International Botanical Congess

Abstract Number: 5844
Poster No. = 2198


ANTISENSE TRANSFORMANTS OF CND41, A CHLOROPLAST DNA-BINDING PROTEIN OFTOBACCO, SHOWED INCREASES IN CHLOROPLAST GENE EXPRESSIONS AND DECREASE IN GIBBERELLIN SYNTHESIS.


Takeshi Nakano1, Takuma Kimura1, Noriko Nagata1, Shinya Murakami2, Masayo Sekimoto3, Hiroshi Kawaide3, Yuji Kamiya3, Fumihiko Sato2 and Shigeo Yoshida1 1 Plant Functions Lab., 3 FRP, The Inst. of Phys. and Chem. Res. (RIKEN), Wako 351-01, Japan. 2 (B Dept. Plant Gene and Totipotency, Kyoto Univ., Kyot o 606-01. Japan.


CND41, a chloroplast DNA-binding protein was considered as arepressor for chloroplast gene expressions. CND41 antisense transformants showed a higher level of expression for psbA, rbcL, and 16S/23S rRNA compared to wild-type, and also showed trends in dwarfism. CND41 mRNA expression were not effected by cytokinin, gibberellin and brassinolide in leaf tissues. The dwarf phenotype of stem and shorten phenotype of leaves in the transformants were recovered by feeding of GA3 on shoot apex. Analysis of gibberellin (GA1) contents in leaves of the antisense transformants were lower than wild type. Chloroplast developmental stage in transformants may correlated with the GA1 synthesis.

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