XVI International Botanical Congess
With no recombination, all phylogenetic information from chloroplast DNA (cpDNA) should converge on a single estimate of the 'gene genealogy' for this organellar genome. The extensive cpDNA rearrangements in the Campanulales are initial impediments in Southern blot analysis, but once resolved, these rearrangements constitute a separate class of molecular data that can be independently analyzed and compared with evidence from restriction-site analysis or DNA sequence data. By comparing multiple lines of evidence from a genetic system that yields a single, non-reticulate phylogenetic estimate, one can establish a baseline of expectations for more diverse lines of evidence that are not similarly constrained. A cpDNA-based phylogenetic estimate for the Lobeliaceae is presented and the lines of evidence are compared.