ibclogo XVI International Botanical Congess


Abstract Number: 5823
Poster No. = 503


REARRANGEMENTS, RESTRICTION SITES, AND NUCLEOTIDE SEQUENCES IN LOBELIACEAE CHLOROPLAST DNA: ALL ROADS LEAD TO ROME.


E. B. Knox, Dept. of Biological Sciences, Rutgers University, Newark NJ 07102, USA


With no recombination, all phylogenetic information from chloroplast DNA (cpDNA) should converge on a single estimate of the 'gene genealogy' for this organellar genome. The extensive cpDNA rearrangements in the Campanulales are initial impediments in Southern blot analysis, but once resolved, these rearrangements constitute a separate class of molecular data that can be independently analyzed and compared with evidence from restriction-site analysis or DNA sequence data. By comparing multiple lines of evidence from a genetic system that yields a single, non-reticulate phylogenetic estimate, one can establish a baseline of expectations for more diverse lines of evidence that are not similarly constrained. A cpDNA-based phylogenetic estimate for the Lobeliaceae is presented and the lines of evidence are compared.


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