ibclogo XVI International Botanical Congess


Abstract Number: 5656
Poster No. = 1910


THE SIGNALS LINKED TO FOREIGN GENE B.t-McryIAc TARGET ITS PROTEINS TO ER OF TRANSGENIC PLANTS


Wenyu Yang* and Yongyan Bai#. *Dept. of BioEngineering, Northeast Agrl. Univ., Harbin,150030, # NLPMG, Shanghai Inst. of Plant Physiol., Shanghai, 200032, People's Republic of China


The retention signal seqs. from cDNA encoding ABP-a luminal ER protein were linked to an anti-insect gene B.t-McryIAc. Two A.tumefaciens binary vectors with one (pMcryIAc+ER) containing the complete siganl seqs. and another (pMcryIAc-ER) without 3'-KDEL signal seq. were constructed and introduced into A.t. Transgenic potato plants were obtained from A.t-mediated transformation. Both target genes were integrated into genome and expressed in some transgenic plants. The absence of 3' KDEL-signal of McryIAc-ER results in secretion of its translated proteins from ER but in concentration in the Golgi apparatus when expressed in transgenic plants. Conversely, the proteins from McryIAc+ER were not secreted and instead accumulated in the ER of transgenic plant cells. The results suggest that ER-retention signal seqs. can be used to localize the expression of foreign gene in ER of transgenic plants.


HTML-Version made 7. July 1999 by Kurt Stüber