XVI International Botanical Congess

The present study aimed at identifying the antifungal compound(s) from Japanese birch plantlets infected with a fungus .The Betula platyphylla var. japonica plantlet asceptically grown for 3 months was infected with Inonotus obliquus (Treatment). The uninoculated and uninjured plantlet (Control 1), and uninoculated and injured one (Control 2) were used as controls. After further incubation for one month, these plantlets were deep-frozen, powdered, and extracted with methanol. The extracts were derivatized, and then used for gas chromatograph-mass spectrometer analysis. ß-Sitosterol was identified in the extracts from Treatment, Control 1, and Control 2 by the analysis. By a comparison of peak intensity in total ion chromatograms, the amount of ß-sitosterol formed in Control 1, Control 2, and Treatment seemed to be a phytoanticipin of Japanese birch plantlet. The accurate quantification and antifungal test of this compound are now in progress.