ibclogo XVI International Botanical Congess

Abstract Number: 5648
Poster No. = 2153


CALLUS GROWTH AND PLANT REGENERATION INDUCED BY PLANT GROWTH REGULATORS ON GRACILARIOPSIS TENUIFRONS (GRACILARIALES, RHODOPHYTA).


Nair S. Yokoya, Instituto de Botânica, SMA, São Paulo, C.P. 4005, 001061-970, São Paulo, Brazil


Axenic cultures of Gracilariopsis tenuiforns (Gracilariales, Rhodophyta) were established in ASP 12-NTA solid medium (0.4% agar and 1.0% sucrose). Indole-3-acetic acid (IAA)), 2,4-dichlorophenoxyacetic acid (2, 4-D), and 6-benzylaminopurine (BA) were added individually or in combination (IAA:BA). Calluses we3re originated from divisions of apical anc cortical cells located at apical regions of thallus segments and branches. Low concentration of IAA (0.5 mg.L1) promoted callus formation, while IAA:BA (1.0:1.0 mg.L-1) and 2,4-D (o.5 mg.L-1) stimulate growth of calluses form apical and intercalary segments, respectively, Regeneration of plantlets from calluses was stimulated by BA. These results suggest that auxin and cytokinin are involved in developmental regulatory processes in red algae.

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