XVI International Botanical Congess

The banding patterns of enzyme TPI are frequently complicated in the starch gel electrophoresis of plants. The unexpected bands were often misinterpreted as extra loci. During staining TPI of the samples of Juncus, we found that the slower group of bands came out slightly later than the faster one and was completely the same with the ADH banding pattern which stained with the different slice of the same gel. Moreover, the ADH bands could still come out perfectly even stained it without substrate ethanol in the recipe. When we run the gel with the buffer system of Lithium-borate, pH 8.0/Tris-citrate, pH 7.6 instead of Sodium-borage, pH 8.6/Tris-citrate, pH 7.8, the extra ADH bands did not appear in TPI staining. It was proved that ADH also appear on other NAD-dehydrogenase gels as stain artifacts (nothing dehydrogenases) in plants.