ibclogo XVI International Botanical Congess


Abstract Number: 5544
Poster No. = 476


CLONING AND MOLECULAR EVOLUTION OF A CYCLOIDEA-LIKE GENE IN ARABIDOPSIS


S. Vazquez-Santana and T. P. Holtsford, University of Missouri-Columbia.


The cycloidea gene is involved in the control of the floral asymmetry in Antirrhinum, however, nothing is known in species with symmetrical flowers as Arabidopsis. The goal of this work is to clone homologous genes to cycloidea in different species and ecotypes of Arabidopsis to study the evolutionary rate of these genes in these taxa. Actually, we have cloned using PCR a cycloidea-like gene (cyc-like) from Arabidopsis. The cyc-like DNA contains an open reading frame that encode to a protein of 337 amino acids. In addition, cyc-like gene has around 46% of homology at the amino acid level with proteins branched-1 from teosinte and cycloidea from Antirrhinum. Finally, we are cloning the cyc-like gene from different taxa of Arabidopsis to build a phylogenetic tree. Additionally, we are performing Northern-blot assays to know if this gene is expressed in floral organs.


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