XVI International Botanical Congess

The fresh leaves of subtrib Micheliinae were collected as materials including 14 species from 3 genera.All of the OD260/OD280 values of the total DNA isolated by CTAB method and purificated by Glass-milk kit were around 1.8. 13 primers selected from 3 Operon RAPD kits(A,C and E) were able to amplify good RAPD products. The dendrogram was constructed from genetic distance through UPGMA method.The result show that subtrib Micheliinae can be divided into two genera,genus Paramichelia and Michelia.For genus Michelia ,there are subgenera to be supported.One is subgenus M.figo including M.martinii, M.chapensis,M.yunnanensis and M.figo,the other is subgenus M.alba which is divided into two section-Section M.floribunde including M.floribunda, M.balansae, M.macclurei,M.crassipes and T.odorum, while Section M.alba including M.alba, M.champaca,M.maudiae and M.foveolata.Based on the RAPD analyses, T.odorum is not supported to be separated from genus Michelia and considered to be a genus which is discordant with Liu Yu-hu*s system.However,it is supported to treat P.baillonii as a a single genus indepent of genus Michelia which is appropriate to Liu*system..Comparing thess results with Nooteboom*s or Dandy*s system,some is accord with the two systems while others are not.So further reseach should be done for discussion of the phylogeny of subtrib Micheliinae.