XVI International Botanical Congess

Plant biotechnology includes the technique tissue culture to clonally produce large numbers of identical individuals at minimal cost. Somatic embryogenesis is the best method for high frequency somatic embryo production in most of the Umbelliferae members. In coriander large number of identical elite plants are produced via Somatic embryogenesis/synthetic seeds. Somatic embryos were induced from MS/SH nutrient medium supplemented with different combinations of NAA/2.4D. The matured somatic embryos were encapsulated in CaC12 & Sodium alginate matrix. These encapsulated asexual embryos were treated in different nutrient medium with different growth hormone combinations for embryo germination. Best conversion results was obtained in half strength MS medium containing ABA (0.05 mg 1-1). The survival of synseeds was about 60% which is somewhat higher than other nutrient combinations. The encapsulated embryos was stored in refrigerator 4?C for 3 weeks. Then beads were recultured to produce plants with roots & shoots simultaneously.