ibclogo XVI International Botanical Congess


Abstract Number: 5332
Poster No. = 2012


IDENTIFICATION OF ACTIN- AND MICROTUBULE-ASSOCIATED PROTEIN FROM TOBACCO BY -2 CELLS


H. Igarassu, S. Sonobe and T. Shimmen, Dept. Life Sci., Fac. Sci., Himeji Inst. Tech., Harima Science Park City, Hyogo, 678-1297, Japan


Physiological studies suggested the interaction of actin filaments and microtubules in various plant cells. Although the presence of factor(s) to connect these two cytoskeletons is expected, studies in this line is sparse. The present study aims at identification of such factor(s), using cultured cells of tobacco. From the crude extract, we obtained a polypeptide (190kDa) which bound to both actin filament and microtubules. This polypeptide localized in spindle and midboy during cell division, but not during interphase.


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