ibclogo XVI International Botanical Congess


Abstract Number: 5173
Session = 7.10.7


THE SPITZENKÖRPER FUNCTIONS AS A MICROTUBULE ORGANIZING CENTER IN HYPHAE OF ALLOMYCES.


Roberson, Robert W. & McDaniel, Dennis P., Department of Plant Biology, Arizona State University, Tempe, AZ, 85287


Elongating hyphae of most fungi contain a well defined Spitzenkörper. The role(s) of the Spitzenkörper in hyphal growth and morphogenesis have long been a mystery. We have shown that hyphae of Allomyces macrogynus (Chytridiomycota) contain a well defined Spitzenkörper and recent data confirmed its role as a microtubule organizing center (MTOC). Using indirect immunofluorescence procedures and laser scanning confocal microscopy (LSCM), we have demonstrated that -tubulin is a component of the Spitzenkörper in A. macrogynus. LSCM of the microtubule (MT) cytoskeleton under control conditions and upon recovery from MT depolymerization has provided further evidence that the Allomyces Spitzenkörper functions as a MTOC. By regulating MT nucleation and distribution, the Spitzenkörper of Allomyces plays a critical role in MT-based intracellular motility and maintaining cytoplasmic organization during hyphal tip growth.


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