ibclogo XVI International Botanical Congess


Abstract Number: 5130
Session = We used tetrad analysis in Arabidopsis to perform the first genome-wide measurement of genetic exchange in individual meiotic cells. This analysis is made possible by the Arabidopsis quartet mutant, which causes the products of pollen meiosis to remain attached. By crossing quartet mutants from the Columbia and Landsberg ecotypes, we generated F1 plants with pollen tetrads that segregate hundreds of genetic markers. Individual tetrads from these plants were used to pollinate genetically marked stigmas, which often generates four plants from each cross. We analyzed recombination, at the genomic level, investigating the controls that regulate crossover number and distribution. Using this method we have determined centromere positions for all five chromosomes and have measured recombination frequencies across the entire genome. More recently, we have found treatments that dramatically increase recombination rates, particularly in heterochromatin.


MANIPULATING RECOMBINATION IN


ARABIDOPSIS: CROSSOVER DISTRIBUTIONSAND CENTROMERE MAPPING


Gregory P. Copenhaver, Kate Nickel and Daphne Preuss, Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago IL


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