ibclogo XVI International Botanical Congess


Abstract Number: 5052
Poster No. = 1290


RESCUING SYMONANTHUS BANCROFTII (SOLANACEAE), A WESTERN AUSTRALIAN NATIVE SPECIES FROM EXTINCTION THROUGH IN VITRO MICROPROPAGATION


Maggie Panaia, E. Bunn, T. Senaratna, K.W. Dixon, K. Sivasithamparam*, Kings Park and Botanic Garden, West Perth 6005, Western Australia, *Department of Soil Science, The University of Western Australia, Nedlands 6907, Western Australia


Shoots of the rare and endangered Symonanthus bancroftii were multiplied on modified 1/2 MS medium. Treatment with potassium citrate:citrate (0.1% w/v for 5 mins) reduced tissue oxidation and leakage of phenols into media. Three-fold shoot multiplication was achieved after four weeks on 1/2 MS 0.5mM K, 0.25mM BA. Successful rooting treatments were IBA and NAA at 0.5/0.5mM (31%) and 1.0/1.0mM (36%) after four weeks. Media supplemented with 3mg L-1 of paclobutrazol increased resistance to desiccation, thereby increasing survival of potted plantlets from 50% (control) to 90% and reducing the acclimatisation period by 75%.


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