XVI International Botanical Congess

Cyclopentylmethyl beta-glucoside (CPEM-G) was prepared as substrate analogue for beta-glucosidases (BGH). Inhibitory activity of CPEM-G for BGH from microorganisms and plants were examined with p-nitrophenyl beta-glucoside (pNP-G) as substrate. CPEM-G had more inhibitory activity for Almond BGH (inhibition constant:0.15mM) than for Trichoderma viride and Aspergillus niger BGH. pNP-G hydrolytic activities of crude enzymes from petals of rose and lily were also inhibited by CPEM-G. When cut ends of lily flowering stalks and rose flowering branches were put in the appropriate concentration of CPEM-G solution, reductions of flower fragrance were observed. It leads to the presumed mechanism that the inhibition of BGH in the flowers reduces the liberation of fragrance from precursors.