XVI International Botanical Congess

A stable genic male sterile line was obtained in Brassica campestris through years' screening of inbred seeds. The fertility segregated in a typical 3:1 ratio, indicating that fertility difference was controlled by a recessed allele. A restore gene type and a sterile gene type were used to created a segregated population for the target gene and bulked-DNA was prepared for screening the single target gene under the same genetic background. Two hundred primers of 10 mr operon were used for RAPD screening. On the genomic DNA from both sterile and fertile plants, among that 200 markers, one specific marker associated with genic male sterile was found. Identification of this marker with genomic DNA from other 100 restore lines and sterile lines further proved that this marker was linked to genomic DNA. The primer was CTGCATCGTG, which was ampling at a specific site (about 720bp) in bulked-DNA from restore lines. The southern blotting results revealed that this marker fragment is single copy, its sequence is 58% homogeneous to Bp4 gene, a pollen-specific gene family from Brassica napus which is activated during early microspore development. It included the promoter and TATA signal for Bp4 gene.