ibclogo XVI International Botanical Congess

Abstract Number: 4884
Poster No. = 1907


DELAYED AND SUPPRESSED NECROTIC LESION FORMATION ON TMV-INFECTED TRANSGENIC TOBACCO PLANTS OVERPRODUCING ANIMAL CELL DEATH SUPPRESSORS.


v I. Mitsuhara1,2, Y. Gotoh1, M. Miura3, Y. Ohashi1,2, 1Natl. Inst. of Agrobiological Resources, Tsukuba 305 Japan, 2JST& CREST, 3Medical School, Osaka Univ., Suita 565 Japan


We have generated transgenic tobacco plants overexpresing bcl-xL or ced-9, animal cell death suppressor genes. Transgenic plants resisted against UV and paraquat treatment depending on the amount of the BCL-xL and Ced-9 protein. Further N gene-dependent necrotic lesion formation in TMV-inoculated tobacco leaves was suppressed. In a synchronous lesion-formation system, appearance time of lesions was delayed about 2h and levels of ion leakage associated lesion formation was suppressed, arrwing enhanced TMV-multiplication than wild-type plant. These data suggested that animal cell death suppressors could inhibit hypersensitive response of plants.

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