ibclogo XVI International Botanical Congess

Abstract Number: 4786
Session = Role of the phytohormones in plant differentiation, including reproduction system, is discussed.


PHYTOHORMONAL MUTANTS IN INBRED RADISH LINES MODEL SYSTEM FOR DIFFERENTIATION OF PLANTS


L. A. Lutova*, T. V. Matveeva*, I. E. Dodueva*, I. S. Buzovkina*, O.N. Tikhodeev* and E. Nester^, *Dpt. of Genetics and Breeding, St.Petersburg State University, Russia, ^Dpt. of Microbiology, University of Washington, Seattle, USA.


Our work is devoted to the molecular genetic analysis of the genes involved in determination of differentiation processes in higher plants. As a unique model genetic collection of highly inbred radish lines (36 generations of inbreeding) was screened for in vivo or in vitro differentiation mutants. Among typical in vivo mutant phenotypes known to be related to certain defects in plant phytohormonal system (i.e. dwarfism, giantism, wiltiness and abnormal geotropism), we have also found the new mutants able to produce spontaneous genetic tumors and changing of flower meristem determination. We have shown that some radish lines may be considered as a new type of phytohormonal mutants defective in some components of the auxin/cytokinin regulation system. Preliminary evidence based on Southern analysis suggests that both tumor and non-tumor lines contain sequences similar to the tmr and 6b (tml ) genes, but not the tms genes of T-DNA.

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