XVI International Botanical Congess

Seventeen Asian species of Lindsaea were investigated cytologically. The basic numbers are x=44 and 47 in most species, except in L. odorata complex, in which there are 2n=c.302 chromosomes in L. odorata var. odorata and n=c.75, 2n=c.150 chromosomes in var. japonica in which L. odorata var. odorata has 2n=c.302 and var. japonica has n=c.75, 2n=c.150. The basic number of L. odorata complex may be x=75. Further, the cytological data and morphological characters suggested the hybrid origin of L. simulans (meiosis irregular and 2n=c.174, 4x) and L. heterophylla (2n=c.130, 216, 3x, 5x?), and a triploid hybrid (2n=228) in L. odorata complex. For examining the putative hybrids and compared with ideas on relationship evolved from morphological and cytological investigations, Molecular systematic studies (isozame electropholesis and RAPD analysis) were carried out to test the above hypothesis. The results verified that L. simulans originated from intraspecific hybridization of L. chienii (4x) and L. orbiculata (4x), L. heterophylla originated from crossing between L. orbiculata and L. ensifolia (4x), and the triploid hybrid var. odorata x var. japonica probably from interspecific hybridization.