ibclogo XVI International Botanical Congess

Abstract Number: 4697
Poster No. = 1845


SUGAR BEET MITOCHONDRIAL GENOME CONTAINS RED USED SIZED RPS4: NUCLEOTIDE SEQUENCE AND RNA EDITING.


Tomohiko Kubo, Noriko Itchoda and Tetsuo Mikami, Fac. Agriculture, Hokkaido University, Sapporo, Japan.


Ribosomal protein S4 gene (rps4) in angiosperm mitochondria is interesting because it harbors the extra 160 amino acids in the center of the ORF compared to liverwort. The role(s) of the extra residues are unknown, although it is conserved in rapeseed, Arabidopsis and rice. When we cloned rps4 from the mitochondrial genome of sugar beet (Beta vulgaris. L), we found that sugar beet rps4 consists of 315 amino acids, which is much shorter than that of rapeseed (362 a. a.) and rice (352 a. a.) due to the deletion in the central region. cDNA analysis of rps4 revealed that 10 C-to-U editings are occurred on the mRNA, leading us to conclude that sugar beet rps4 is the functional gene. This suggests that some part of the angiosperm-specific central region is dispensable.

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