ibclogo XVI International Botanical Congess


Abstract Number: 4631
Session = 4.18.2


INITIATION OF DIFFERENT ORGANS CULTURES OF HYPERICUM PERFORATUM AND OBTAINING OF HYPERICIN.


A.B.Kirakosyan, H.R. Vardapetian, A.G. Tchartchoglian, *A.A. Charchoglian, Yerevan State University, Dept. of Biophysics, *Inst. of Botany, National Academy of Sciences of Armenia.


Plant cell biotechnology for the production of secondary metabolites particularly increasing yield metabolic engineering seems to be a promising approach, but requires the understanding of the regulation of secondary metabolism at all its levels: genes, enzymes, transport, compartmentation and products. For a stable producing cell line is in most cases satisfactory if there is taken into account some aspects: molecular biological regulation, growth of differentiated cells, elisitation, epigenetic manipulation, screening and selection. The ability for synthesis hypericin by suspension cell and tissue cultures from leaf, stamens and petals of H.perforatum L. was investigated. The highest hypericin content was found suspensions derived from stamens of H.perforatum collected in Sevan. Adapted suspension cell culture three kinds of cell populations with different content of hypericin were identified and selected. The morphocytological peculiarities and hypericin content of the selected cell populations was investigated. Cell aggregates proved to be excellent producers of some secondary metabolites not found in cell suspension cultures. Selection of high-producing strains as well as experiments to improve the product yields in these strains are in progress. Production of secondary metabolites in plants is often connected with morphological differentiation.


HTML-Version made 7. July 1999 by Kurt Stüber