XVI International Botanical Congess

The production of plants with new useful properties and resistance to various pathogens is one of basic directions of modern plant biotechnology. Now, flax is the object of genetical engineering, but eachcultivar needs it is own individual conditions of transformation. we transformed 4 domestic cultivar flax: Belinka, Dashkovsky-2, Aleksin and A-29. Segments of 7-10 day-old aseptic hypocotyls were used in capacity of explants. The explants of flax were transformed with Agrobacterium tumefaciens LBA4404(pAL4404,pGA482) and A.tumefaciens LBA4404(pAL4404,pGA482::M.EcoRII). The callus lines were grown on selective medium MS, containing 1,0 mg/l 6-bensiaminopurine,0,1 mg/l napthalene acetic acid 100 mg/l kanamycin for a long time. The efficiency of transformation depends on flax cultivars and Agrobacterium strain. The efficiency of transformation of all cultivars with A. tumefaciens LBA4404(pAL4404,pGA482::M.EcoRII) was less than in the case with an empty vector. The activity of neomycin phosphotransferase II was demonstrated for kanamycin resistant callus lines (KmR). Molecular biological studies of the obtained KmR callus lines are in progress.