ibclogo XVI International Botanical Congess


Abstract Number: 4534
Poster No. = 1671


ACTIN DYNAMICS IN LIVING EGG APPARATUS CELLS BEFORE AND AFTERFERTILIZATION IN TORENIA FOURNIERI


Y. Fu2, M. Yuan3, B-Q Huang1, H-Y Yang2 and S .Y. Zee1, 1The University of Hong Kong, 2Wuhan University, 3China Agricultural University


The architecture and dynamics of actin cytoskeleton play important roles in plant embryo sacs during fertilization. Our results clearly show that the formation of actin coronas results from the dynamic changes of actin cytoskeleton in the synergid, the egg cell and discharged contents of the pollen tube. Coupling with degeneration after anthesis, actin filaments in synergids turned into numerous aggregates, which initiating the formation of an actin corona. While in the egg cell, short F-actin bundles distributing randomly in the cortex were organized into a network. An F-actin cap-like structure formed at the filiform apparatus, which would disappear after the arrival of the pollen tube. After pollination F-actin relatively increased in the degenerated synergid and at intercellular space between the female germ unit cells, while in the egg cell actin filaments depolymerized into patches. Those actin components were spatially organized together to form coronas.


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