ibclogo XVI International Botanical Congess


Abstract Number: 4462
Poster No. = 60


THE RIBOSOMAL CISTRON AND RED ALGAL RELATIONSHIPS: THE UTILITY OF LARGE-SUBUNIT RIBOSOMAL DNA SEQUENCE DATA


James T. Harper and Gary W. Saunders, University of New Brunswick, Fredericton, N.B., Canada, E3B 6E1


Sequences of the ribosomal cistron have been useful as molecular tools for inferring organismal phylogenies. Within the Rhodophyta, sequences of the small-subunit have had the greatest impact at the ordinal level. However, now that virtually all known lineages of red algae have been explored, a number of relationships remain unresolved. Our focus is now on the potential of sequences from the large-subunit (LSU) for addressing the phylogenetics of the Rhodophyta. The LSU is quite large and possesses a number of regions of varying divergence. These features should provide the resolution not obtained from more conservative molecular data sets. At present we have generated sequence data for ca. 80% of the LSU for over 35 members of the Rhodophyta, spanning virtually all the recognized florideophyte orders. Comparative SSU and LSU phylogenies will be presented, a critical evaluation of the LSU as an informative molecular tool will be given, and future directions for LSU research will be discussed.


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